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xcite r 120led fluorescence illuminator  (Carl Zeiss)


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    Carl Zeiss xcite r 120led fluorescence illuminator
    Xcite R 120led Fluorescence Illuminator, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xcite+120led/fluorescence+illuminator/pmc11513987__pnas__2403906121__sapp-71-15-5
    Average 90 stars, based on 1 article reviews
    xcite r 120led fluorescence illuminator - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: HLH-2/E2A Expression Links Stochastic and Deterministic Elements of a Cell Fate Decision during C. elegans Gonadogenesis
    Article Snippet: .. Long-term imaging was performed on a Zeiss AxioObserver.Z1 inverted microscope with a 40X, 1.4NA oil immersion objective, a 26mm WD, 0.55NA condenser and an XCite 120LED for epifluorescence microscopy illumination. .. Images were captured with a Hamamatsu Orca flash 4.0 LT+ CMOS camera.

    Inverted Microscopy:

    Article Title: HLH-2/E2A Expression Links Stochastic and Deterministic Elements of a Cell Fate Decision during C. elegans Gonadogenesis
    Article Snippet: .. Long-term imaging was performed on a Zeiss AxioObserver.Z1 inverted microscope with a 40X, 1.4NA oil immersion objective, a 26mm WD, 0.55NA condenser and an XCite 120LED for epifluorescence microscopy illumination. .. Images were captured with a Hamamatsu Orca flash 4.0 LT+ CMOS camera.

    Epifluorescence Microscopy:

    Article Title: HLH-2/E2A Expression Links Stochastic and Deterministic Elements of a Cell Fate Decision during C. elegans Gonadogenesis
    Article Snippet: .. Long-term imaging was performed on a Zeiss AxioObserver.Z1 inverted microscope with a 40X, 1.4NA oil immersion objective, a 26mm WD, 0.55NA condenser and an XCite 120LED for epifluorescence microscopy illumination. .. Images were captured with a Hamamatsu Orca flash 4.0 LT+ CMOS camera.



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    A. Diagram of genetic strategy for generating Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; <t>Ai80</t> (RCFL-CatCh)-D and Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D mice. Isl1 Cre +/- mice were crossed with Slc17a6 FlpO +/+ mice to drive the expression of Cre and FlpO recombinase in <t>dI3</t> neurons and Vglut2 + nociceptive afferents, forming dI3-driver mice. For optogenetic experiments, Isl1 Cre +/- ; Slc17a6 FlpO +/+ (dI3-driver) mice were crossed with Ai80 (RCFL-CatCh)-D (CatCh) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the CatCh/EYFP fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D <t>(dI3-driver-CatCh)</t> mice. To visualize the dI3 neurons and Vglut2 + afferents, dI3-driver mice were crossed with Ai65 (RCFL-tdT)-D (TdTomato) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the TdTomato fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D (dI3-driver-TdTomato) mice. B. Representative image of TdTomato (red) labelled dI3 neurons and Vglut2 + afferents in dI3-driver-TdTomato mice. To avoid activation of these afferents in dI3-driver-CatCh mice, a dorsal hemisection was performed at the indicated level. C. Diagram of the ex vivo spinal cord preparation. The spinal cord was isolated in neonatal P1-P7 dI3-driver-CatCh mice, and a dorsal hemisection was performed at the cervical (C1-C8) and lumbar (L1-L5) segments to remove Vglut2-CatCh + afferents. Three suction electrodes were simultaneously applied to various ventral roots to record from C4-C8 and L1-L5 segments, and blue light was applied to ipsilateral segments. Within the same animals, blue light was also independently applied to contralateral segments from C4-C8 and L1-L5.
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    A. Diagram of genetic strategy for generating Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; <t>Ai80</t> (RCFL-CatCh)-D and Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D mice. Isl1 Cre +/- mice were crossed with Slc17a6 FlpO +/+ mice to drive the expression of Cre and FlpO recombinase in <t>dI3</t> neurons and Vglut2 + nociceptive afferents, forming dI3-driver mice. For optogenetic experiments, Isl1 Cre +/- ; Slc17a6 FlpO +/+ (dI3-driver) mice were crossed with Ai80 (RCFL-CatCh)-D (CatCh) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the CatCh/EYFP fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D <t>(dI3-driver-CatCh)</t> mice. To visualize the dI3 neurons and Vglut2 + afferents, dI3-driver mice were crossed with Ai65 (RCFL-tdT)-D (TdTomato) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the TdTomato fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D (dI3-driver-TdTomato) mice. B. Representative image of TdTomato (red) labelled dI3 neurons and Vglut2 + afferents in dI3-driver-TdTomato mice. To avoid activation of these afferents in dI3-driver-CatCh mice, a dorsal hemisection was performed at the indicated level. C. Diagram of the ex vivo spinal cord preparation. The spinal cord was isolated in neonatal P1-P7 dI3-driver-CatCh mice, and a dorsal hemisection was performed at the cervical (C1-C8) and lumbar (L1-L5) segments to remove Vglut2-CatCh + afferents. Three suction electrodes were simultaneously applied to various ventral roots to record from C4-C8 and L1-L5 segments, and blue light was applied to ipsilateral segments. Within the same animals, blue light was also independently applied to contralateral segments from C4-C8 and L1-L5.
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    Image Search Results


    A. Diagram of genetic strategy for generating Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D and Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D mice. Isl1 Cre +/- mice were crossed with Slc17a6 FlpO +/+ mice to drive the expression of Cre and FlpO recombinase in dI3 neurons and Vglut2 + nociceptive afferents, forming dI3-driver mice. For optogenetic experiments, Isl1 Cre +/- ; Slc17a6 FlpO +/+ (dI3-driver) mice were crossed with Ai80 (RCFL-CatCh)-D (CatCh) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the CatCh/EYFP fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D (dI3-driver-CatCh) mice. To visualize the dI3 neurons and Vglut2 + afferents, dI3-driver mice were crossed with Ai65 (RCFL-tdT)-D (TdTomato) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the TdTomato fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D (dI3-driver-TdTomato) mice. B. Representative image of TdTomato (red) labelled dI3 neurons and Vglut2 + afferents in dI3-driver-TdTomato mice. To avoid activation of these afferents in dI3-driver-CatCh mice, a dorsal hemisection was performed at the indicated level. C. Diagram of the ex vivo spinal cord preparation. The spinal cord was isolated in neonatal P1-P7 dI3-driver-CatCh mice, and a dorsal hemisection was performed at the cervical (C1-C8) and lumbar (L1-L5) segments to remove Vglut2-CatCh + afferents. Three suction electrodes were simultaneously applied to various ventral roots to record from C4-C8 and L1-L5 segments, and blue light was applied to ipsilateral segments. Within the same animals, blue light was also independently applied to contralateral segments from C4-C8 and L1-L5.

    Journal: bioRxiv

    Article Title: Mapping of dI3 neuron sensorimotor circuits across the cervical and lumbar spinal cord

    doi: 10.1101/2024.11.17.624039

    Figure Lengend Snippet: A. Diagram of genetic strategy for generating Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D and Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D mice. Isl1 Cre +/- mice were crossed with Slc17a6 FlpO +/+ mice to drive the expression of Cre and FlpO recombinase in dI3 neurons and Vglut2 + nociceptive afferents, forming dI3-driver mice. For optogenetic experiments, Isl1 Cre +/- ; Slc17a6 FlpO +/+ (dI3-driver) mice were crossed with Ai80 (RCFL-CatCh)-D (CatCh) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the CatCh/EYFP fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D (dI3-driver-CatCh) mice. To visualize the dI3 neurons and Vglut2 + afferents, dI3-driver mice were crossed with Ai65 (RCFL-tdT)-D (TdTomato) mice containing a frt -flanked STOP cassette and a loxP -flanked STOP cassette upstream of the TdTomato fluorescent protein, resulting in Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai65 (RCFL-tdT)-D (dI3-driver-TdTomato) mice. B. Representative image of TdTomato (red) labelled dI3 neurons and Vglut2 + afferents in dI3-driver-TdTomato mice. To avoid activation of these afferents in dI3-driver-CatCh mice, a dorsal hemisection was performed at the indicated level. C. Diagram of the ex vivo spinal cord preparation. The spinal cord was isolated in neonatal P1-P7 dI3-driver-CatCh mice, and a dorsal hemisection was performed at the cervical (C1-C8) and lumbar (L1-L5) segments to remove Vglut2-CatCh + afferents. Three suction electrodes were simultaneously applied to various ventral roots to record from C4-C8 and L1-L5 segments, and blue light was applied to ipsilateral segments. Within the same animals, blue light was also independently applied to contralateral segments from C4-C8 and L1-L5.

    Article Snippet: Stimulation of dI3 neurons was performed using the Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D (dI3-driver-CatCh) mice and blue light (X-Cite 120 LEDMini; 470 nm).

    Techniques: Expressing, Activation Assay, Ex Vivo, Isolation

    A. Optogenetic stimulation of ipsilateral lumbar dI3s evokes fictive locomotion in flexor and extensor roots. (i) and (iii) Experimental setup and sample locomotor recordings. Spinal cord preparations consisted of isolated lumbar spinal cords from neonatal dI3-driver-CatCh mice with a dorsal hemisection. Suction electrodes were applied to (i) L1 and L4 (red) and (iii) L2 and L5 (blue) roots while optogenetically stimulating ipsilateral L1-L5 segments (L3 not shown). (ii and iv) Blue light was applied for 10 seconds continuously, and alternating, rhythmic activity was observed in both recording electrodes during the stimulation period.

    Journal: bioRxiv

    Article Title: Mapping of dI3 neuron sensorimotor circuits across the cervical and lumbar spinal cord

    doi: 10.1101/2024.11.17.624039

    Figure Lengend Snippet: A. Optogenetic stimulation of ipsilateral lumbar dI3s evokes fictive locomotion in flexor and extensor roots. (i) and (iii) Experimental setup and sample locomotor recordings. Spinal cord preparations consisted of isolated lumbar spinal cords from neonatal dI3-driver-CatCh mice with a dorsal hemisection. Suction electrodes were applied to (i) L1 and L4 (red) and (iii) L2 and L5 (blue) roots while optogenetically stimulating ipsilateral L1-L5 segments (L3 not shown). (ii and iv) Blue light was applied for 10 seconds continuously, and alternating, rhythmic activity was observed in both recording electrodes during the stimulation period.

    Article Snippet: Stimulation of dI3 neurons was performed using the Isl1 Cre +/- ; Slc17a6 FlpO +/+ ; Ai80 (RCFL-CatCh)-D (dI3-driver-CatCh) mice and blue light (X-Cite 120 LEDMini; 470 nm).

    Techniques: Isolation, Activity Assay